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Protection of pigs against experimental Salmonella Typhimurium infection by use of a single dose subunit slow delivery vaccine

机译：通过使用单剂量亚单位缓释疫苗来保护猪免受实验性鼠伤寒沙门氏菌感染

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Infections caused by septicemic strains of Salmonella are significant animal health as well as food safety concerns for the North American swine industry. Among the various strategies to control these infections at the herd level, development of vaccines are attractive alternatives. In this study, based on previous studies of immune response to various protems following natural and experimental infections of pigs by Salmonella, we designed a subunit slow delivery vaccine and tested it in an experimental model of infection. The selected immunogenic protein was cloned and purified by chromatography. The purified protein was then incorporated m PLGA (a polymer that is slowly degraded within the animal\u27s gastro-intestinal system) microspheres and given orally once to groups of pigs (n=8) while control animals (n=8) received only PBS. Animals were challenged orally 4 weeks after the vaccmation with 108 cells of a virulent strains of Salmonella Typhimurium. Animals were examined twice a day and climcal signs evaluated using a predetermined scoring grid. Pigs were sacrificed 12 days later and bacterial cultures of vanous organs, electron microscopy and evaluation of lgA response by ELISA were performed. No significant difference was found at bacteriology and ELISA but marked differences in clinical signs were observed between vaccinated and non vaccinated animals. None of vaccmated animals showed fever exceeding 40°C while it was observed in 5 out of 8 non vaccinated Only one of vaccmated pigs showed mild diarrhea while severe diarrhea was observed in all control animals different sizes of microspheres were observed in intestinal crypts of vaccinated animals at electron microscopy. We concluded that this vaccine can protect pigs against clinical signs associated with experimental infection by Salmonella Typhimunum.
机译：沙门氏菌败血症菌株引起的感染是重要的动物健康以及北美养猪业的食品安全问题。在畜群一级控制这些感染的各种策略中，疫苗的开发是有吸引力的选择。在这项研究中，基于先前对沙门氏菌自然和实验性感染猪后对各种蛋白质的免疫反应的研究，我们设计了亚单位慢递送疫苗，并在感染的实验模型中对其进行了测试。克隆选择的免疫原性蛋白质，并通过色谱法纯化。然后将纯化的蛋白质掺入PLGA（一种在动物胃肠系统中缓慢降解的聚合物）微球中，并口服给予一组猪（n = 8）一次，而对照动物（n = 8）仅接受PBS 。接种鼠伤寒沙门氏菌的强毒株108细胞后4周，对动物进行口服攻击。每天两次检查动物，并使用预定的评分网格评估其爬坡症状。 12天后将猪处死，并进行动物脏器细菌培养，电子显微镜和通过ELISA评估IgA应答。在细菌学和ELISA中没有发现显着差异，但是在接种疫苗的动物和未接种疫苗的动物之间观察到了明显的临床体征。接种疫苗的动物均未显示出超过40°C的发烧，而在未接种疫苗的8个动物中，有5例观察到发烧在电子显微镜下。我们得出的结论是，这种疫苗可以保护猪免受与伤寒沙门氏菌感染相关的临床症状。

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